Reflectance confocal microscopy for noninvasive monitoring of therapy and detection of subclinical actinic keratoses

Dermatology. 2010;220(1):15-24. doi: 10.1159/000254893. Epub 2009 Nov 11.

Abstract

Background: Actinic keratoses (AK) represent cutaneous carcinoma in situ and have previously been evaluated by reflectance confocal microscopy (RCM). Treatment of AK with imiquimod (IMIQ) 5% cream has been shown to 'highlight' subclinical lesions.

Objective: The aim of this study was to test the applicability of RCM for noninvasive monitoring of actinic field cancerization and detection of subclinical AK.

Subjects and methods: AK and surrounding skin sites with no apparent AK of 11 volunteers were selected for imaging and subsequently classified as 'clinical' and 'subclinical' AK. IMIQ was used 3 times weekly for 4 weeks.

Results: RCM was able to detect morphologic features of AK in both clinical and subclinical AK; features were more pronounced in clinical lesions. The immunomodulatory response induced by IMIQ was visualized by RCM.

Conclusion: Our findings indicate that RCM allows noninvasive monitoring of treatment response in vivo and permits early detection of subclinical AK, thus substantiating the incentive for therapy.

Publication types

  • Evaluation Study

MeSH terms

  • Aged
  • Aminoquinolines / therapeutic use*
  • Antineoplastic Agents / therapeutic use*
  • Carcinoma in Situ / drug therapy*
  • Carcinoma in Situ / pathology
  • Drug Monitoring / methods*
  • Humans
  • Imiquimod
  • Keratosis, Actinic / drug therapy*
  • Keratosis, Actinic / pathology
  • Microscopy, Confocal / methods*
  • Middle Aged
  • Treatment Outcome

Substances

  • Aminoquinolines
  • Antineoplastic Agents
  • Imiquimod