Performance of Elecsys toxo IgG and IgM immunoassays

Clin Lab. 2009;55(7-8):267-73.

Abstract

Roche Diagnostics has developed two new rapid and fully automated assays for the detection of Anti-Toxo-IgG and Toxo-IgM antibodies from human sera and plasma. The performance evaluation of the Elecsys Toxo IgG resulted in a sensitivity of 100% and a specificity of 99.91% showing that this assay allows a very sensitive and specific detection of Toxo IgG antibodies with an excellent discrimination of positive and negative results. The performance evaluation of the Elecsys Toxo IgM assay revealed a sensitivity of 100% and a specificity of 99.11% after resolution. Less reactivity towards persistent Toxo IgM antibodies in samples from Toxoplasma infections > 3 months was found with Elecsys Toxo IgM. The performance evaluation data demonstrate that the Elecsys Toxo IgG and Elecsys Toxo IgM assays are reliable tools in routine diagnostics of Toxoplasma infections with the additional advantage of a high throughput on fully automated analyzers MODULAR ANALYTICS Serum Work Area.

MeSH terms

  • Animals
  • Antibodies, Protozoan / blood
  • Antigens, Surface / blood
  • Antigens, Surface / immunology
  • Automation / methods
  • Calibration
  • Humans
  • Immunoassay / methods*
  • Immunoglobulin G / blood*
  • Immunoglobulin M / blood*
  • Sensitivity and Specificity
  • Toxoplasma / immunology*
  • Toxoplasmosis / blood
  • Toxoplasmosis / diagnosis
  • Toxoplasmosis / immunology*

Substances

  • Antibodies, Protozoan
  • Antigens, Surface
  • Immunoglobulin G
  • Immunoglobulin M