Advancement of a multiplex PCR for the differentiation of all currently described Brucella species

Anne Mayer-Scholl; Angelika Draeger; Cornelia Göllner; Holger C Scholz; Karsten Nöckler

doi:10.1016/j.mimet.2009.10.015

Advancement of a multiplex PCR for the differentiation of all currently described Brucella species

J Microbiol Methods. 2010 Jan;80(1):112-4. doi: 10.1016/j.mimet.2009.10.015. Epub 2009 Nov 1.

Authors

Anne Mayer-Scholl¹, Angelika Draeger, Cornelia Göllner, Holger C Scholz, Karsten Nöckler

Affiliation

¹ Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, 12277 Berlin, Germany. anne.mayer-scholl@bfr.bund.de

PMID: 19887090
DOI: 10.1016/j.mimet.2009.10.015

Abstract

To facilitate routine laboratories in the effective diagnosis of brucellosis, we report a robust and rapid multiplex PCR assay, which allows for the differentiation of all nine currently recognised Brucella species. This includes the recently described species B. microti, B. inopinata, B. ceti and B. pinnipedialis.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Brucella / classification
Brucella / genetics
Brucella / isolation & purification*
Brucellosis / diagnosis
Brucellosis / microbiology*
Humans
Polymerase Chain Reaction / methods*