FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka; Sho-hei Fujishima; Sho-hei Uchinomiya; Akio Ojida; Itaru Hamachi

doi:10.1016/j.bmcl.2009.09.122

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Bioorg Med Chem Lett. 2009 Dec 1;19(23):6696-9. doi: 10.1016/j.bmcl.2009.09.122. Epub 2009 Oct 3.

Authors

Hiroshi Nonaka¹, Sho-hei Fujishima, Sho-hei Uchinomiya, Akio Ojida, Itaru Hamachi

Affiliation

¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.

PMID: 19837586
DOI: 10.1016/j.bmcl.2009.09.122

Abstract

A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

MeSH terms

Binding Sites
Models, Molecular
Molecular Structure
Oligopeptides
Peptides / chemistry*
Proteins / chemistry*
Staining and Labeling

Substances

Oligopeptides
Peptides
Proteins
FLAG peptide