Impaired synthesis of erythropoietin, glutamine synthetase and metallothionein in the skin of NOD/SCID/gamma(c)(null) and Foxn1 nu/nu mice with misbalanced production of MHC class II complex

Neurochem Res. 2010 Jun;35(6):899-908. doi: 10.1007/s11064-009-0074-x. Epub 2009 Oct 14.

Abstract

Most skin pathologies are characterized by unbalanced synthesis of major histocompatability complex II (MHC-II) proteins. Healthy skin keratinocytes simultaneously produce large amounts of MHC-II and regeneration-supporting proteins, e.g. erythropoietin (EPO), EPO receptor (EPOR), glutamine synthetase (GS) and metallothionein (MT). To investigate the level of regeneration-supporting proteins in the skin during misbalanced production of MHC-II, skin sections from nonobese diabetic/severe combined immunodeficient (NOD/SCID)/gamma (c) (null) and or Foxn1 nu/nu mice which are a priory known to under- and over-express MHC II, respectively, were used. Double immunofluorescence analysis of NOD/SCID/gamma (c) (null) skin sections showed striking decrease in expression of MHC-II, EPO, GS and MT. In Foxn1 nu/nu mouse skin, GS was strongly expressed in epidermis and in hair follicles (HF), which lacked EPO. In nude mouse skin EPO and MHC-II were over-expressed in dermal fibroblasts and they were completely absent from cortex, channel, medulla and keratinocytes surrounding the HF, suggest a role for EPO in health and pathology of hair follicle. The level of expression of EPO and GS in both mutant mice was confirmed by results of Western blot analyses. Strong immunoresponsiveness of EPOR in the hair channels of NOD/SCID/gamma (c) (null) mouse skin suggests increased requirements of skin cells for EPO and possible benefits of exogenous EPO application during disorders of immune system accompanied by loss MHC-II in skin cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Erythropoietin / biosynthesis*
  • Fluorescent Antibody Technique
  • Forkhead Transcription Factors / genetics
  • Glutamate-Ammonia Ligase / biosynthesis*
  • Histocompatibility Antigens Class II / biosynthesis*
  • Interleukin Receptor Common gamma Subunit / genetics
  • Metallothionein / biosynthesis*
  • Mice
  • Mice, Inbred NOD
  • Mice, Knockout
  • Mice, SCID
  • Microscopy, Confocal
  • Skin / metabolism*
  • Species Specificity

Substances

  • Forkhead Transcription Factors
  • Histocompatibility Antigens Class II
  • Il2rg protein, mouse
  • Interleukin Receptor Common gamma Subunit
  • Whn protein
  • Erythropoietin
  • Metallothionein
  • Glutamate-Ammonia Ligase