Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

W J B van Wamel; S Hansenová Manásková; A C Fluit; H Verbrugh; A J de Neeling; E van Duijkeren; A van Belkum

doi:10.1007/s10096-009-0816-3

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398

Eur J Clin Microbiol Infect Dis. 2010 Jan;29(1):119-22. doi: 10.1007/s10096-009-0816-3. Epub 2009 Oct 2.

Authors

W J B van Wamel¹, S Hansenová Manásková, A C Fluit, H Verbrugh, A J de Neeling, E van Duijkeren, A van Belkum

Affiliation

¹ ErasmusMC, Department of Medical Microbiology and Infectious Diseases, University Medical Centre Rotterdam, Rotterdam, The Netherlands. w.vanwamel@erasmusmc.nl

Abstract

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

Publication types

Evaluation Study

MeSH terms

Animals
Bacterial Typing Techniques / methods*
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field
Evolution, Molecular*
Genotype
Humans
Methicillin Resistance
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Staphylococcal Infections / microbiology*
Staphylococcal Infections / veterinary*
Staphylococcus aureus / classification*
Staphylococcus aureus / genetics*
Staphylococcus aureus / isolation & purification

Substances

DNA, Bacterial