Abstract
Optimal conditions for the assessment of T cell proliferation and cytolytic T lymphocyte (CTL) responses specific for primate lentivirus were established. CTL and T cell proliferative responses were demonstrated as early as two weeks post-infection. Although the majority of CTL were CD8+, MHC class I-restricted, this study demonstrated CD4+, MHC class II-restricted CTL. Experiments also demonstrated that CTL were CD16 negative. Target cell generation could be blocked with CD4 mAb specific for the SIV binding site.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / blood
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Blotting, Western
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CD4-Positive T-Lymphocytes / immunology
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Cell Division
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Female
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Histocompatibility Antigens Class II / immunology
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Macaca mulatta
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Male
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Phenotype
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Simian Acquired Immunodeficiency Syndrome / immunology*
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Simian Immunodeficiency Virus / immunology*
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T-Lymphocytes / immunology*
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Regulatory / immunology
Substances
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Antibodies, Viral
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Histocompatibility Antigens Class II