Alpha-1-adrenergic receptors in the nucleus tractus solitarii region of rats with experimental and genetic hypertension

Brain Res. 1990 Jun 11;519(1-2):261-5. doi: 10.1016/0006-8993(90)90087-r.

Abstract

The binding of the alpha 1-adrenergic receptors antagonist, 125I-HEAT, to membranes of nucleus tractus solitarii (NTS) regions of the brains of neurogenic hypertensive, spontaneously hypertensive (SH), and deoxycorticosterone (DOCA)/salt hypertensive rats and their respective controls was studied to quantitate the expression of alpha 1-adrenergic receptors. Scatchard analysis of the binding studies revealed a 1.8-fold increase in the Bmax of alpha 1-adrenergic receptors in NTS region membranes of SH rats when compared to their Wistar-Kyoto (WKY) control without significant difference in the Kd for 125I-HEAT. A decrease in the Kd with no difference in Bmax of receptors for 125I-HEAT was observed in the NTS region membranes of neurogenic hypertensive rats when compared with their sham-operated controls. In contrast, comparison of the Bmax and Kd values for 125I-HEAT binding in NTS region membrane of the DOCA/salt hypertensive rats and its sham-operated control showed no significant differences. We suggest that alterations in baroreceptor afferent activity may be related to alterations in central alpha 1-adrenergic receptors binding in SH rats and rats with neurogenic hypertension.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenergic alpha-Antagonists / metabolism
  • Afferent Pathways / physiology
  • Animals
  • Blood Pressure
  • Cell Membrane / metabolism
  • Desoxycorticosterone
  • Hypertension / chemically induced
  • Hypertension / genetics
  • Hypertension / metabolism*
  • Kinetics
  • Medulla Oblongata / metabolism*
  • Phenethylamines / metabolism
  • Pressoreceptors / physiology
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred Strains
  • Rats, Inbred WKY
  • Receptors, Adrenergic, alpha / metabolism*
  • Reference Values
  • Tetralones*

Substances

  • Adrenergic alpha-Antagonists
  • Phenethylamines
  • Receptors, Adrenergic, alpha
  • Tetralones
  • BE 2254
  • Desoxycorticosterone