Trypsin-gold nanoparticle conjugates: binding, enzymatic activity, and stability

Prep Biochem Biotechnol. 2009;39(4):429-38. doi: 10.1080/10826060903210024.

Abstract

Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20 nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Enzyme Activation
  • Enzyme Stability
  • Gold / chemistry*
  • Materials Testing
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure*
  • Particle Size
  • Protein Binding
  • Trypsin / chemistry*
  • Trypsin / ultrastructure*

Substances

  • Gold
  • Trypsin