Production and secretion of adiponectin from 3T3-L1 adipocytes: comparison of antihypertensive drugs

Am J Hypertens. 2009 Oct;22(10):1126-9. doi: 10.1038/ajh.2009.171. Epub 2009 Sep 3.

Abstract

Background: Adiponectin is an important vascular protective adipocytokine that possesses antidiabetic, antiatherogenic, and anti-inflammatory properties. The aim of this study was to evaluate the effect of various antihypertensive drugs on the production and secretion of adiponectin from adipocytes.

Methods: 3T3-L1 adipocytes were incubated for 6 h with increased doses of the following drugs: hydrochlorothiazide, atenolol, losartan, telmisartan, captopril, and nifedipine. Adiponectin levels, as well as adiponectin-mRNA expression, were measured in the medium and cells.

Results: Significant increases of adiponectin were induced by telmisartan: 56% with a dose of 0.1 micromol/l (P < 0.05), 131% with 10 micromol/l (P < 0.05), and 125% with 100 micromol/l (P < 0.01). Losartan (100 micromol/l) also increased adiponectin by 65% (P < 0.05). Conversely, hydrochlorothiazide, 0.1 micromol/l, reduced adiponectin by 37% (P < 0.01). Captopril, atenolol, and nifedipine had no effect on adiponectin. Gene expression of adiponectin correlated with these results: with telmisartan, it increased by 27%, and with hydrochlorothiazide it decreased by 38% (P < 0.05 for both compared to the control).

Conclusion: In this comparative model, telmisartan, and to a lesser extent, losartan, increased production and secretion of adiponectin from 3T3-L1 adipocytes compared to the other antihypertensive drugs.

Publication types

  • Comparative Study

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Adiponectin / genetics
  • Adiponectin / metabolism*
  • Animals
  • Antihypertensive Agents / pharmacology*
  • Atenolol / pharmacology
  • Benzimidazoles / pharmacology
  • Benzoates / pharmacology
  • Captopril / pharmacology
  • Hydrochlorothiazide / pharmacology
  • Losartan / pharmacology
  • Mice
  • Nifedipine / pharmacology
  • RNA, Messenger / metabolism
  • Telmisartan

Substances

  • Adiponectin
  • Antihypertensive Agents
  • Benzimidazoles
  • Benzoates
  • RNA, Messenger
  • Hydrochlorothiazide
  • Atenolol
  • Captopril
  • Nifedipine
  • Losartan
  • Telmisartan