An Mrp-like cluster in the halotolerant cyanobacterium Aphanothece halophytica functions as a Na+/H+ antiporter

Appl Environ Microbiol. 2009 Oct;75(20):6626-9. doi: 10.1128/AEM.01387-09. Epub 2009 Aug 21.

Abstract

The mrp homolog gene cluster mrpCD1D2EFGAB (Ap-mrp) was found in a halotolerant cyanobacterium, Aphanothece halophytica, amplified, and expressed in Escherichia coli mutant TO114. Ap-mrp complemented the salt-sensitive phenotype of TO114 and exhibited Na(+)/H(+) and Li(+)/H(+) exchange activities, indicating that Ap-Mrp functions as a Na(+)/H(+) antiporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Cyanobacteria / genetics*
  • Cyanobacteria / growth & development
  • Cyanobacteria / metabolism*
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Multigene Family*
  • Mutation
  • Phenotype
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Salinity
  • Sodium-Hydrogen Exchangers / genetics*
  • Sodium-Hydrogen Exchangers / metabolism*
  • Synechococcus / genetics
  • Synechococcus / growth & development
  • Synechococcus / metabolism

Substances

  • Bacterial Proteins
  • DNA Primers
  • DNA, Bacterial
  • Recombinant Proteins
  • Sodium-Hydrogen Exchangers

Associated data

  • GENBANK/AB507743