High-Q polymer-coated microspheres for immunosensing applications

Opt Express. 2009 Aug 17;17(17):14694-9. doi: 10.1364/oe.17.014694.

Abstract

Homogeneous polymeric thin layers have been used as functionalizing agents on silica microspherical resonators in view of the implementation of an immunosensor. We have characterized the microspheres functionalized with poly-L-lactic acid and Eudragit L100, as an alternative to the commonly used 3-Aminopropyltrimethoxysilane. It is shown that polymeric functionalization does not affect the high quality factor (Q greater than 10(7)) of the silica microspheres, and that the Q factor is about 3 x 10(5) after chemical activation and covalent binding of immunogammaglobulin (IgG). This functionalizing process of the microresonator constitutes a promising step towards the achievement of an ultra sensitive immunosensor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques*
  • Equipment Design
  • Humans
  • Immune System / metabolism
  • Immunoglobulin G / chemistry*
  • Lactic Acid / chemistry*
  • Microscopy, Atomic Force / methods
  • Microspheres*
  • Optics and Photonics
  • Particle Size
  • Polyesters
  • Polymers / chemistry*
  • Polymethacrylic Acids / chemistry*
  • Protein Binding
  • Silicon Dioxide / chemistry*

Substances

  • Immunoglobulin G
  • Polyesters
  • Polymers
  • Polymethacrylic Acids
  • methylmethacrylate-methacrylic acid copolymer
  • Lactic Acid
  • poly(lactide)
  • Silicon Dioxide