Abstract
A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alphavirus Infections / diagnosis*
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Chikungunya virus / genetics
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Chikungunya virus / isolation & purification*
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Humans
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Molecular Sequence Data
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RNA, Viral / genetics
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Reagent Kits, Diagnostic
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Viral Load
Substances
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RNA, Viral
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Reagent Kits, Diagnostic