Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum

J R Bright; R Mackness; M J Danson; D W Hough; G L Taylor; P Towner; D Byrom

doi:10.1016/0022-2836(91)90200-p

Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum

J Mol Biol. 1991 Nov 20;222(2):143-4. doi: 10.1016/0022-2836(91)90200-p.

Authors

J R Bright¹, R Mackness, M J Danson, D W Hough, G L Taylor, P Towner, D Byrom

Affiliation

¹ Department of Biochemistry, University of Bath Claverton Down, U.K.

PMID: 1960718
DOI: 10.1016/0022-2836(91)90200-p

Abstract

Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 angstrom, c = 229.6 angstrom and with two molecules in the asymmetric unit.

MeSH terms

Bacterial Proteins / ultrastructure*
Crystallography
Glucose 1-Dehydrogenase
Glucose Dehydrogenases / ultrastructure*
Protein Conformation
Thermoplasma / enzymology*
X-Ray Diffraction

Substances

Bacterial Proteins
Glucose Dehydrogenases
Glucose 1-Dehydrogenase