Abstract
Twenty-five (27 %) of 92 clinically normal macaques were found to have beta-haemolytic Escherichia coli isolated from their faeces. Five of six isolates chosen for further characterization had multiple antibiotic resistance and were PCR-positive for cytotoxic necrotizing factor 1 (cnf1) with a demonstrated cytopathic effect in vitro. By repetitive element sequence-based PCR genotyping, genetic similarity was established for selected isolates. We believe this to be the first report of E. coli strains producing CNF1 in non-human primates.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Bacterial Toxins / biosynthesis*
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Bacterial Toxins / genetics
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DNA Fingerprinting
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Escherichia coli / classification
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Escherichia coli / genetics
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Escherichia coli / isolation & purification*
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Escherichia coli / pathogenicity
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Escherichia coli Proteins / biosynthesis*
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Escherichia coli Proteins / genetics
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Feces / microbiology
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Genes, Bacterial
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HeLa Cells
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Humans
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Macaca fascicularis / microbiology*
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Macaca mulatta / microbiology*
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Serotyping
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Virulence / genetics
Substances
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Bacterial Toxins
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DNA, Bacterial
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Escherichia coli Proteins
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cytotoxic necrotizing factor type 1