Expanding RNA silencing approaches by U1 adaptors

Chembiochem. 2009 Jul 6;10(10):1599-601. doi: 10.1002/cbic.200900271.

Abstract

Silent partner: In a recent publication, Gunderson and co-workers described an approach to recruit the abundant U1 snRNP, a splicing subparticle, to the last exon of a pre-mRNA, in a sequence-specific manner. This mediates interaction of the U1-70K protein subunit with poly(A) polymerase, thus blocking polyadenylation and inducing pre-mRNA degradation. This novel promising strategy expands the repertoire of gene-silencing concepts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • HeLa Cells
  • Humans
  • Polynucleotide Adenylyltransferase / genetics
  • Polynucleotide Adenylyltransferase / metabolism
  • RNA Interference*
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • Ribonucleoprotein, U1 Small Nuclear / metabolism*

Substances

  • RNA Precursors
  • Ribonucleoprotein, U1 Small Nuclear
  • Polynucleotide Adenylyltransferase