Comparability of ELISA and toxin neutralization to measure immunogenicity of Protective Antigen in mice, as part of a potency test for anthrax vaccines

P M Parreiras; L A Sirota; L D Wagner; S L Menzies; J L Arciniega

doi:10.1016/j.vaccine.2009.05.045

Comparability of ELISA and toxin neutralization to measure immunogenicity of Protective Antigen in mice, as part of a potency test for anthrax vaccines

Vaccine. 2009 Jul 16;27(33):4537-42. doi: 10.1016/j.vaccine.2009.05.045. Epub 2009 Jun 6.

Authors

P M Parreiras¹, L A Sirota, L D Wagner, S L Menzies, J L Arciniega

Affiliation

¹ Oswaldo Cruz Foundation, Research Center Rene Rachou, Av. Augusto de Lima 1715, Belo Horizonte, MG 30190-002, Brazil.

PMID: 19501205
DOI: 10.1016/j.vaccine.2009.05.045

Abstract

Complexities of lethal challenge models have prompted the investigation of immunogenicity assays as potency tests of anthrax vaccines. An ELISA and a lethal toxin neutralization assay (TNA) were used to measure antibody response to Protective Antigen (PA) in mice immunized once with either a commercial or a recombinant PA (rPA) vaccine formulated in-house. Even though ELISA and TNA results showed correlation, ELISA results may not be able to accurately predict TNA results in this single immunization model.

Publication types

Comparative Study

MeSH terms

Animals
Anthrax / immunology
Anthrax / prevention & control*
Anthrax Vaccines / immunology*
Antibodies, Bacterial / immunology
Antigens, Bacterial / immunology*
Bacterial Toxins / immunology*
Enzyme-Linked Immunosorbent Assay
Female
Mice
Neutralization Tests
Recombinant Proteins / immunology
Vaccines, Synthetic / immunology

Substances

Anthrax Vaccines
Antibodies, Bacterial
Antigens, Bacterial
Bacterial Toxins
Recombinant Proteins
Vaccines, Synthetic
anthrax toxin