The relation of adiponectin and tumor necrosis factor alpha levels between endothelial nitric oxide synthase, angiotensin-converting enzyme, transforming growth factor beta, and tumor necrosis factor alpha gene polymorphism in adrenal incidentalomas

J Endocrinol Invest. 2009 Dec;32(11):881-8. doi: 10.1007/BF03345766. Epub 2009 May 12.

Abstract

Objective: The aim of our study was to demonstrate demographic characteristics, presence of inflammatory markers, distribution of angiotensin-converting enzyme (ACE), tumor necrosis factor (TNF), endothelial nitric oxide synthase (eNOS) genotypes and relations among these parameters in these patients and control subjects.

Research design and methods: Study samples were collected from 50 patients with adrenal mass and 30 control groups. The eNOS, ACE, TNF-alpha, transforming growth factor (TGF)-beta genes polymorphisms, TNF-alpha, adiponectin levels were analysed in 50 unrelated Turkish patients with a diagnosis of adrenal incidentaloma (AI).

Results: There was statistically significant difference between TNF-alpha levels of patient and controls (p=0.048). We have not detected the connection between TGF-beta, TNF-alpha, ACE, eNOS gene polymorphism with serum TNF-alpha and adiponectin levels. In this study, we demonstrated that there were significant differences for ACE genotypes in the patients when compared to the controls (p<0.05). The percentages of the ID, DD, II genotypes for ACE gene polymorphism in the patients group were 30.0, 13.0, 7.0%, respectively.

Conclusions: According to different cases of eNOS, TGF-beta, ACE, and TNF-alpha gene genotypes; no statistical significant difference was found between basal cortisol, ACTH, DHEAS, metanephrine, renin, aldosterone, normetanephrine, 17-hydroxyprogesterone, 1 mg low-dose dexamethasone suppression test-cortisol response and AI size. In this study, I/D genotype was determined to be statistically higher in ACE gene in patients with AI (p=0.014).

MeSH terms

  • Adiponectin / blood*
  • Adrenal Gland Neoplasms / genetics*
  • Aged
  • Body Mass Index
  • Female
  • Genotype
  • Haplotypes
  • Humans
  • Incidental Findings
  • Male
  • Middle Aged
  • Nitric Oxide Synthase Type III / genetics*
  • Peptidyl-Dipeptidase A / genetics*
  • Polymorphism, Genetic
  • Transforming Growth Factor beta / genetics*
  • Tumor Necrosis Factor-alpha / genetics*

Substances

  • Adiponectin
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • NOS3 protein, human
  • Nitric Oxide Synthase Type III
  • Peptidyl-Dipeptidase A