When placed in a novel environment, mice tend to explore for a period of time, and then reduce the level of exploration. This reduction in locomotor or exploratory behavior is known as habituation and can occur within a single session or across sessions, respectively, termed intrasession and intersession habituation. Recent research indicates that there is a genetic component to habituation behavior and that some of the genes involved differ between the two types of habituation. The genetic evidence also suggests that intrasession habituation and intersession habituation are measuring somewhat different conceptual entities and with more such evidence may eventually help us understand the different pathways involved. Some of the genetic methods and tools used to unravel the roles of specific genes in both types of habituation are outlined here, with examples from the literature, as well as new data, to illustrate that this seemingly simple behavior is actually very complicated in terms of genetics. Evidence to date suggests that a number of genetic regions play roles in one or both types of habituation, and further research will be necessary to determine the specific genes involved.