Human papillomavirus genotype specificity of hybrid capture 2 low-risk probe cocktail

J Clin Microbiol. 2009 Aug;47(8):2611-5. doi: 10.1128/JCM.00278-09. Epub 2009 Jun 3.

Abstract

A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak, were due to cross-reactivity with high-risk genotypes. Establishment of a gray zone is recommended.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Cross Reactions
  • DNA, Viral / genetics*
  • DNA, Viral / isolation & purification*
  • Female
  • Genotype
  • Humans
  • Nucleic Acid Hybridization / methods*
  • Oligonucleotide Probes / genetics
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • DNA, Viral
  • Oligonucleotide Probes