Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam (Mercenaria mercenaria)

Appl Environ Microbiol. 2009 Jul;75(14):4913-8. doi: 10.1128/AEM.00246-09. Epub 2009 May 22.

Abstract

We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • DNA Primers / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Geologic Sediments / parasitology
  • Mercenaria / parasitology*
  • Molecular Sequence Data
  • Parasites / classification
  • Parasites / genetics
  • Parasites / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Time Factors

Substances

  • DNA Primers
  • DNA, Ribosomal

Associated data

  • GENBANK/FJ533155
  • GENBANK/FJ533156
  • GENBANK/FJ533157
  • GENBANK/FJ533158
  • GENBANK/FJ533159
  • GENBANK/FJ533160
  • GENBANK/FJ533161
  • GENBANK/FJ533162
  • GENBANK/FJ533163