A synthetic NCAM-derived mimetic peptide, FGL, exerts anti-inflammatory properties via IGF-1 and interferon-gamma modulation

J Neurochem. 2009 Jun;109(5):1516-25. doi: 10.1111/j.1471-4159.2009.06076.x.

Abstract

Microglial cell activity increases in the rat hippocampus during normal brain aging. The neural cell adhesion molecule (NCAM)-derived mimetic peptide, FG loop (FGL), acts as an anti-inflammatory agent in the hippocampus of the aged rat, promoting CD200 ligand expression while attenuating glial cell activation and subsequent pro-inflammatory cytokine production. The aim of the current study was to determine if FGL corrects the age-related imbalance in hippocampal levels of insulin-like growth factor-1 (IGF-1) and pro-inflammatory interferon-gamma (IFNgamma), and subsequently attenuates the glial reactivity associated with aging. Administration of FGL reversed the age-related decline in IGF-1 in hippocampus, while abrogating the age-related increase in IFNgamma. FGL robustly promotes IGF-1 release from primary neurons and IGF-1 is pivotal in FGL induction of neuronal Akt phosphorylation and subsequent CD200 ligand expression in vitro. In addition, FGL abrogates both age- and IFNgamma-induced increases in markers of glial cell activation, including major histocompatibility complex class II (MHCII) and CD40. Finally, the proclivity of FGL to attenuate IFNgamma-induced glial cell activation in vitro is IGF-1-dependent. Overall, these findings suggest that FGL, by correcting the age-related imbalance in hippocampal levels of IGF-1 and IFNgamma, attenuates glial cell activation associated with aging. These findings also highlight a novel mechanism by which FGL can impact on neuronal CD200 ligand expression and subsequently on glial cell activation status.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Animals, Newborn
  • Anti-Inflammatory Agents / pharmacology*
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • CD40 Antigens / genetics
  • CD40 Antigens / metabolism
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism*
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism*
  • Male
  • Neural Cell Adhesion Molecules / pharmacology*
  • Neuroglia / drug effects*
  • Neuroglia / metabolism
  • Neurons / drug effects*
  • Neurons / metabolism
  • Oncogene Protein v-akt / genetics
  • Oncogene Protein v-akt / metabolism
  • Rats
  • Rats, Wistar

Substances

  • Anti-Inflammatory Agents
  • Antigens, CD
  • CD40 Antigens
  • NCAM protein (681-695), human
  • Neural Cell Adhesion Molecules
  • Insulin-Like Growth Factor I
  • Interferon-gamma
  • Oncogene Protein v-akt
  • antigens, CD200