Abstract
Epitope vaccine is a promising option for therapeutic vaccination against Helicobacter pylori (H. pylori) infection. In this study, we constructed a multi-epitope vaccine with five epitopes and mucosal adjuvant E. coli heat-labile enterotoxin B subunit (LTB) named HUepi-LTB and evaluated its therapeutic effect against H. pylori infection in BALB/c mice model. HUepi-LTB containing three Th epitopes from UreB and two B cell epitopes from UreB and HpaA was constructed and expressed in E. coli. Oral therapeutic immunization with HUepi-LTB significantly decreased H. pylori colonization compared with oral immunization with PBS, and the protection was correlated with antigen-specific CD4+ T cells and IgG and mucosal IgA antibody responses. This multi-epitope vaccine may be a promising vaccine candidate that may help to control H. pylori infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / pharmacology
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Administration, Oral
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Animals
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Antibodies, Bacterial / immunology
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Bacterial Toxins / administration & dosage
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Bacterial Toxins / pharmacology
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology*
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CD4-Positive T-Lymphocytes / immunology
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Enterotoxins / administration & dosage
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Enterotoxins / pharmacology
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / immunology*
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology*
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Escherichia coli / genetics
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Escherichia coli Proteins / administration & dosage
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Escherichia coli Proteins / pharmacology
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Female
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Helicobacter Infections / immunology
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Helicobacter Infections / prevention & control*
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Helicobacter Infections / therapy*
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Helicobacter pylori / genetics
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Helicobacter pylori / immunology*
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Humans
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Immunity, Mucosal
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Immunoglobulin A / immunology
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Immunoglobulin G / immunology
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Mice
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Mice, Inbred BALB C
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Bacterial Toxins
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Bacterial Vaccines
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Enterotoxins
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Epitopes, B-Lymphocyte
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Epitopes, T-Lymphocyte
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Escherichia coli Proteins
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Immunoglobulin A
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Immunoglobulin G
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Vaccines, Synthetic
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heat-labile enterotoxin, E coli