Therapeutic efficacy of a multi-epitope vaccine against Helicobacter pylori infection in BALB/c mice model

Vaccine. 2009 Aug 6;27(36):5013-9. doi: 10.1016/j.vaccine.2009.05.009. Epub 2009 May 27.

Abstract

Epitope vaccine is a promising option for therapeutic vaccination against Helicobacter pylori (H. pylori) infection. In this study, we constructed a multi-epitope vaccine with five epitopes and mucosal adjuvant E. coli heat-labile enterotoxin B subunit (LTB) named HUepi-LTB and evaluated its therapeutic effect against H. pylori infection in BALB/c mice model. HUepi-LTB containing three Th epitopes from UreB and two B cell epitopes from UreB and HpaA was constructed and expressed in E. coli. Oral therapeutic immunization with HUepi-LTB significantly decreased H. pylori colonization compared with oral immunization with PBS, and the protection was correlated with antigen-specific CD4+ T cells and IgG and mucosal IgA antibody responses. This multi-epitope vaccine may be a promising vaccine candidate that may help to control H. pylori infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adjuvants, Immunologic / pharmacology
  • Administration, Oral
  • Animals
  • Antibodies, Bacterial / immunology
  • Bacterial Toxins / administration & dosage
  • Bacterial Toxins / pharmacology
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • Enterotoxins / administration & dosage
  • Enterotoxins / pharmacology
  • Epitopes, B-Lymphocyte / genetics
  • Epitopes, B-Lymphocyte / immunology*
  • Epitopes, T-Lymphocyte / genetics
  • Epitopes, T-Lymphocyte / immunology*
  • Escherichia coli / genetics
  • Escherichia coli Proteins / administration & dosage
  • Escherichia coli Proteins / pharmacology
  • Female
  • Helicobacter Infections / immunology
  • Helicobacter Infections / prevention & control*
  • Helicobacter Infections / therapy*
  • Helicobacter pylori / genetics
  • Helicobacter pylori / immunology*
  • Humans
  • Immunity, Mucosal
  • Immunoglobulin A / immunology
  • Immunoglobulin G / immunology
  • Mice
  • Mice, Inbred BALB C
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology

Substances

  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Bacterial Toxins
  • Bacterial Vaccines
  • Enterotoxins
  • Epitopes, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • Escherichia coli Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Vaccines, Synthetic
  • heat-labile enterotoxin, E coli