This study was to verify the performance of three different collimators that were equipped to the clinical application type of small semiconductor gamma camera (SSGC) for radio-guided surgery. We also wanted to see if the clinical application type could be effective to detect sentinel nodes in simulation studies for sentinel lymph node biopsy. The camera head consisted of a pixelized CdTe module (32 x 32 individual elements, total of 1,024 pixels) (Acrorad Co. Ltd., Tokyo, Japan). The field of view was 44.8 mm x 44.8 mm. The clinical application type of this gamma camera had three exchangeable collimators; standard, high sensitivity and high resolution (ST, HS, HR). Energy resolution, full-width at half-maximum (FWHM), of the CdTe detector attached with the standard collimator was 6.9% at 141 keV (99mTc). The spatial resolution, represented by FWHM, had a mean value of 1.59 mm. The data was comparable to the results of the prototype SSGC. The simulation studies showed that HS could more sensitively detect the simulated nodes than ST and HR did, and HR could more reliably distinguish the simulated sentinel node that situated close to the injection site than other two collimators did. However the depiction was interfered by the higher background radiation levels. We suggest that this SSGC clinical application type may provide advantages over the standard system for isolating sentinel lymph nodes biopsy. We also believe that the SSGC may aid surgeons in identifying target tissues when performing radio-guided surgery.