Abstract
A new polymerase chain reaction-restriction fragment length polymorphism method for the identification of cry8-type genes from Bacillus thuringiensis has been established by designing a pair of new universal primers. By this method, a novel gene, cry8Ga1, encoding a polypeptide of 1,157 amino acids with a deduced molecular mass of 131.2 kDa was identified and cloned from B. thuringiensis HBF-18. Recombinant B. thuringiensis strain HD8G, harboring cry8Ga1, has insecticidal activity against larvae of Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. This is the first report of a Cry toxin that has insecticidal activity to Melolonthidae pest H. oblita.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacillus thuringiensis / genetics*
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Bacillus thuringiensis / metabolism*
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Bacillus thuringiensis Toxins
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / toxicity*
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Cloning, Molecular
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Coleoptera / drug effects*
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DNA Primers / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Endotoxins / chemistry
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Endotoxins / genetics*
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Endotoxins / toxicity*
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Hemolysin Proteins / chemistry
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Hemolysin Proteins / genetics*
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Hemolysin Proteins / toxicity*
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Larva / drug effects
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Lethal Dose 50
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Molecular Sequence Data
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Molecular Weight
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Polymerase Chain Reaction / methods
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Sequence Analysis, DNA
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Survival Analysis
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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DNA Primers
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DNA, Bacterial
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Endotoxins
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Hemolysin Proteins
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insecticidal crystal protein, Bacillus Thuringiensis