Objective: To study the rationality of extraction prosess for the Ligusticum chuanxiong in Hongye Xintong Soft Capsule by super critical fluid extraction(SFE).
Method: Conditions for the extraction were optimized by orthogonal experimental design as guided by the extraction rate and content of ligustilid in the extract; Combined with the experiment of rats ligated the left coronary artery, the two compounds containing different extracts (steam distillation and SFE) were compared to determine the extraction prosess.
Result: The best extraction conditions were established as following: pressure 30 MPa, temperature 50 degrees C, extracting time 4 h, separate pressure 10 MPa. separate temperature 40 degrees C. Compared with steam distillation, the compound containing SFE extract showed stronger protective effects on rats ligated the left coronary artery.
Conclusion: Super critical fluid can extract active ingredients in Ligusticum chuanxiong effectively.