In vivo imaging of oligonucleotidic aptamers

Bertrand Tavitian; Frédéric Ducongé; Raphaël Boisgard; Frédéric Dollé

doi:10.1007/978-1-59745-557-2_15

In vivo imaging of oligonucleotidic aptamers

Methods Mol Biol. 2009:535:241-59. doi: 10.1007/978-1-59745-557-2_15.

Authors

Bertrand Tavitian¹, Frédéric Ducongé, Raphaël Boisgard, Frédéric Dollé

Affiliation

¹ Laboratoire d Imagerie Moléculaire Expérimentale, Service hospitalier Frédéric joliot, Intitut d Imagerie Biomédicale, Orsay, France.

PMID: 19377991
DOI: 10.1007/978-1-59745-557-2_15

Abstract

In this chapter we present the methods developed in our laboratory for in vivo imaging of oligonucleotidic aptamers. These methods relate to (i) the labelling of aptamers with fluorine-18, a positron emitter, (ii) Positron Emission Tomography imaging of laboratory animals with [(18)F]aptamers and (iii) labelling with fluorescent dyes and optical imaging of aptamers in mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aptamers, Nucleotide / blood
Aptamers, Nucleotide / chemistry*
Aptamers, Nucleotide / metabolism
Fluorescence
Fluorescent Dyes
Fluorodeoxyglucose F18 / blood
Fluorodeoxyglucose F18 / chemistry*
Fluorodeoxyglucose F18 / metabolism
Mice
Positron-Emission Tomography / methods*
Rats
Whole Body Imaging / methods*

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Fluorodeoxyglucose F18