Abstract
Cognitive enhancement by the GABA (B) antagonist SGS742 has been reported and we decided to search for proteins involved. Hippocampi from OF1 mice were from SGS742- treated animals and three control groups. Proteins were extracted and run on 2DE, and spots were quantified. Significantly different protein spots were identified by two mass spectrometry principles, nano-LC-ESI-MS/MS and by nano-LC-ESI(CID/ETD)-MS/MS. Signaling, chaperone and metabolic enzyme proteins were linked to memory enhancement.
MeSH terms
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Animals
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Cognition*
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Electrophoresis, Gel, Two-Dimensional
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Gene Expression Profiling
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Gene Expression Regulation*
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Hippocampus / metabolism*
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Mice
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Molecular Chaperones / metabolism
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Neuroprotective Agents / pharmacology
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Organophosphorus Compounds / pharmacology
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Proteins / chemistry*
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Proteomics / methods*
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Signal Transduction
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Spectrometry, Mass, Electrospray Ionization
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Molecular Chaperones
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Neuroprotective Agents
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Organophosphorus Compounds
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Proteins
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(3-aminopropyl)(n-butyl)phosphinic acid