Drug resistance and genotypic analysis of Mycobacterium tuberculosis strains from Thai tuberculosis patients

APMIS. 2009 Apr;117(4):286-90. doi: 10.1111/j.1600-0463.2009.02438.x.

Abstract

The aim of this study was the molecular characterization of primary drug-resistant Mycobacterium tuberculosis strains in Thailand. We examined a group of M. tuberculosis isolates from newly registered tuberculosis (TB) cases, collected at the largest university hospital, the Siriraj Hospital, in Thailand. Of 76 selected drug-resistant M. tuberculosis strains recovered from previously untreated pulmonary TB patients whose sputum samples were sent to this hospital, 29 (38%) were single-drug resistant, 26(34%) multidrug resistant and two (2.6%) extensively drug resistant. Fifty (66%) strains belonged to Beijing genotype. The study demonstrated a severe problem of drug resistance among recently detected TB patients, and two large clusters of genetically similar strains indicated ongoing transmission of drug-resistant TB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antitubercular Agents / pharmacology*
  • Child
  • Child, Preschool
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial*
  • Female
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / isolation & purification
  • Polymerase Chain Reaction
  • Retrospective Studies
  • Sequence Analysis, DNA
  • Thailand / epidemiology
  • Tuberculosis / drug therapy
  • Tuberculosis / epidemiology
  • Tuberculosis / microbiology*
  • Young Adult

Substances

  • Antitubercular Agents
  • DNA, Bacterial