Multiplex polymerase chain reaction (PCR) and real-time multiplex PCR for the simultaneous detection of plant viruses

V Pallas; J Sanchez-Navarro; A Varga; F Aparicio; D James

doi:10.1007/978-1-59745-062-1_16

Multiplex polymerase chain reaction (PCR) and real-time multiplex PCR for the simultaneous detection of plant viruses

Methods Mol Biol. 2009:508:193-208. doi: 10.1007/978-1-59745-062-1_16.

Authors

V Pallas¹, J Sanchez-Navarro, A Varga, F Aparicio, D James

Affiliation

¹ IBMCP, Universidad Politecnica de Valencia-Consejo Superior de Investigaciones Cientificas, CPI, Valencia, Spain.

PMID: 19301757
DOI: 10.1007/978-1-59745-062-1_16

Abstract

Multiplex Polymerase Chain Reaction (PCR) can be used for the simultaneous detection of plant viruses. Multiple primer pairs or polyvalent primer pairs can be used to detect and identify several viruses in a single PCR.

MeSH terms

Clinical Laboratory Techniques
DNA Primers
DNA, Viral / analysis*
Fluorescence
Plant Viruses / genetics
Plant Viruses / isolation & purification*
Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / methods*

Substances

DNA Primers
DNA, Viral