Identification of Escherichia coli and Salmonella enterica organisms with reduced susceptibility to ceftriaxone from fecal samples of cows in dairy herds

Am J Vet Res. 2009 Mar;70(3):389-93. doi: 10.2460/ajvr.70.3.389.

Abstract

Objective: To estimate the relationship between therapeutic use of ceftiofur and recovery of Escherichia coli and Salmonella spp with reduced susceptibility to ceftriaxone from feces of dairy cattle.

Animals: 3,840 mature dairy cows on 50 dairy herds in Ohio. Procedures-Fecal samples were obtained from up to 100 mature dairy cows on each farm. Samples were screened for E coli and Salmonella spp with reduced susceptibility to ceftriaxone by use of selective media.

Results: E coli with reduced susceptibility to ceftriaxone was recovered from 92% (46/50) of the herds and 60.9% (2,338/3,840) of cows. Salmonella spp were recovered from 44% (22/50) of the herds and 9.9% (382/3,840) of cows. No association was found between ceftiofur use and recovery of E coli with reduced susceptibility to ceftriaxone at the herd level. However, recovery of E coli with reduced susceptibility to ceftriaxone was more likely from cows in herds in which Salmonella spp were also recovered on the day of collection (odds ratio, 24.96; 95% confidence interval, 3.17 to 196.68) than from herds in which Salmonella spp were not recovered. Odds of recovery of E coli with reduced susceptibility to ceftriaxone from an individual cow increased 62% (odds ratio, 1.62; 95% confidence interval, 1.16 to 2.25) for every 454-kg increase in herd milk production.

Conclusions and clinical relevance: No evidence was found that the use of ceftiofur on dairy farms increases the prevalence or dissemination of Salmonella spp or E coli with reduced susceptibility to ceftriaxone.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Cattle
  • Ceftriaxone / pharmacology*
  • Cephalosporins / pharmacology
  • Drug Resistance, Bacterial*
  • Escherichia coli / drug effects*
  • Escherichia coli / isolation & purification
  • Feces / microbiology*
  • Female
  • Salmonella enterica / drug effects*
  • Salmonella enterica / isolation & purification

Substances

  • Anti-Bacterial Agents
  • Cephalosporins
  • Ceftriaxone
  • ceftiofur