Abstract
We have applied a recently developed label-free mass spectrometry based peptidomic approach to identify and quantify a variety of endogenous peptides from rat nucleus accumbens following withdrawal in naloxone-precipitated, morphine-dependent rats of two separate strains. We focused on maturated, partially processed and truncated peptides derived from the peptide precursors proenkephalin, prodynorphin and preprotachykinin. The expression of several identified peptides was dependent on strain and was affected during morphine withdrawal.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Analgesics, Opioid / metabolism
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Animals
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Chromatography, Liquid / methods
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Humans
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Male
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Molecular Sequence Data
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Morphine / metabolism*
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Naloxone / metabolism*
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Nucleus Accumbens / chemistry*
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Nucleus Accumbens / metabolism
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Opioid Peptides / analysis*
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Protein Precursors / chemistry
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Protein Precursors / metabolism
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Proteome / analysis*
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Rats, Wistar
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Substance Withdrawal Syndrome*
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Tachykinins / chemistry*
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Tachykinins / metabolism
Substances
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Analgesics, Opioid
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Opioid Peptides
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Protein Precursors
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Proteome
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Tachykinins
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Naloxone
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Morphine