ROP2 from Toxoplasma gondii: a virulence factor with a protein-kinase fold and no enzymatic activity

Structure. 2009 Jan 14;17(1):139-46. doi: 10.1016/j.str.2008.11.005.

Abstract

The ROP2 protein and its paralogs are important virulence factors secreted into the host cell by the parasite Toxoplasma gondii. Here we describe the crystal structure of a large and soluble domain of mature ROP2, representative of the ROP2-like protein family. This is a structure of a protein-kinase fold that is devoid of catalytic residues and does not bind ATP. Various structural extensions constitute a signature of this protein family and act to maintain the protein kinase in an open conformation. Our ROP2 structure rules out a previous structural model of attachment of ROP2-like proteins to the parasitophorous vacuole membrane. We propose an alternative mode of membrane attachment implicating basic and amphiphatic helices present in the flexible N terminus of ROP2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Circular Dichroism
  • Cricetinae
  • DNA Primers
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptides / metabolism
  • Protein Folding
  • Protein Kinases / chemistry*
  • Protozoan Proteins / chemistry*
  • Protozoan Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Toxoplasma / chemistry*
  • Toxoplasma / pathogenicity
  • Virulence

Substances

  • DNA Primers
  • Membrane Proteins
  • Peptides
  • Protozoan Proteins
  • ROP 2 protein, Toxoplasma gondii
  • Protein Kinases

Associated data

  • PDB/2W1Z