Object: To establish the fingerprint spectrum of Lonicera macranthoides by HPLC.
Methods: The column of Hypersil gold C18 (4. 6 mm x 250 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.05% phosphoric acid with gradient elution. The column temperature was 30 degrees C, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min.
Results: Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A). There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.
Conclusion: This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides.