Abstract
Objective:
To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.
Methods:
TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.
Results:
TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.
Conclusion:
HBX protein could be induced into mouse liver by TAT induced peptide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Membrane / genetics
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Cell Membrane / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Female
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Gene Expression*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / isolation & purification
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Green Fluorescent Proteins / metabolism
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Hepatitis B / metabolism
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Hepatitis B / virology
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Humans
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Liver / metabolism
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Male
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Mice
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Mice, Inbred ICR
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Protein Transport
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Trans-Activators / genetics
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Trans-Activators / isolation & purification*
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Trans-Activators / metabolism*
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Viral Regulatory and Accessory Proteins / genetics
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Viral Regulatory and Accessory Proteins / isolation & purification*
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Viral Regulatory and Accessory Proteins / metabolism*
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tat Gene Products, Human Immunodeficiency Virus / genetics
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tat Gene Products, Human Immunodeficiency Virus / isolation & purification*
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tat Gene Products, Human Immunodeficiency Virus / metabolism*
Substances
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Recombinant Fusion Proteins
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Trans-Activators
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Viral Regulatory and Accessory Proteins
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enhanced green fluorescent protein
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hepatitis B virus X protein
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tat Gene Products, Human Immunodeficiency Virus
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Green Fluorescent Proteins