[Detection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Aug;22(4):251-3.
[Article in Chinese]

Abstract

Objective: To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas.

Methods: We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well.

Results: 29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.5%. Among the samples detected, 19 were HPV16E6 positive and rate was 61.3%, eight were HPV18 E6 positive and rate was 25.8%; our result also showed five were the multiple infection containing HPV16 and 18 as well and the rate was 71.0%.

Conclusion: The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Linzhou City, Henan Province.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Capsid Proteins / genetics
  • Carcinoma / virology*
  • China
  • DNA Primers / genetics
  • Esophageal Neoplasms / virology*
  • Female
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 16 / isolation & purification*
  • Human papillomavirus 18 / genetics
  • Human papillomavirus 18 / isolation & purification*
  • Humans
  • Male
  • Middle Aged
  • Oncogene Proteins, Viral / genetics
  • Polymerase Chain Reaction

Substances

  • Capsid Proteins
  • DNA Primers
  • HPV L1 protein, Human papillomavirus
  • Oncogene Proteins, Viral