Abstract
This study evaluated the stimulatory effects of machilin A and structurally related lignans isolated from Myristica fragrans on osteoblast differentiation. In two IN VITRO osteoblast differentiation models, machilin A stimulated osteoblast differentiation via activation of p38 MAP kinase. Lignans isolated from Myristica fragrans also stimulated osteoblast differentiation in MC3T3-E1 cells; the lignans included macelignan, machilin F, nectandrin B, safrole, licarin A, licarin B, myristargenol, and meso-dihydroguaiaretic acid. These data suggest that lignans isolated from Myristica fragrans have anabolic activity in bone metabolism.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism
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Animals
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Benzodioxoles / isolation & purification
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Benzodioxoles / pharmacology*
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Bone and Bones / metabolism*
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Cell Differentiation / drug effects*
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Cell Proliferation / drug effects
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Collagen Type I / genetics
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Collagen Type I / metabolism
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Enzyme Inhibitors / pharmacology
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Female
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Gene Expression / drug effects
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Imidazoles / pharmacology
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Lignans / chemistry
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Lignans / isolation & purification
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Lignans / pharmacology*
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Mice
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Myristica / chemistry*
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Osteoblasts / cytology
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Osteoblasts / drug effects*
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Osteocalcin / genetics
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Osteocalcin / metabolism
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Osteopontin / genetics
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Osteopontin / metabolism
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Pyridines / pharmacology
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Seeds
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / genetics*
Substances
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Benzodioxoles
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Collagen Type I
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Enzyme Inhibitors
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Imidazoles
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Lignans
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Plant Extracts
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Pyridines
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Spp1 protein, mouse
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machilin A
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Osteocalcin
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Osteopontin
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p38 Mitogen-Activated Protein Kinases
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Alkaline Phosphatase
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SB 203580