The conserved protein SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size

Dev Cell. 2008 Dec;15(6):901-12. doi: 10.1016/j.devcel.2008.09.018.

Abstract

Microtubules are organized by the centrosome, a dynamic organelle that exhibits changes in both size and number during the cell cycle. Here we show that SZY-20, a putative RNA-binding protein, plays a critical role in limiting centrosome size in C. elegans. SZY-20 localizes in part to centrosomes and in its absence centrosomes possess increased levels of centriolar and pericentriolar components including gamma-tubulin and the centriole duplication factors ZYG-1 and SPD-2. These enlarged centrosomes possess normal centrioles, nucleate more microtubules, and fail to properly direct a number of microtubule-dependent processes. Depletion of ZYG-1 restores normal centrosome size and function to szy-20 mutants, whereas loss of szy-20 suppresses the centrosome duplication defects in both zyg-1 and spd-2 mutants. Our results describe a pathway that determines centrosome size and implicate centriole duplication factors in this process.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Alleles
  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / chemistry
  • Caenorhabditis elegans Proteins / metabolism
  • Caenorhabditis elegans Proteins / physiology*
  • Cell Nucleus / metabolism
  • Centrioles / ultrastructure
  • Centrosome / ultrastructure*
  • Gene Expression Regulation, Developmental*
  • Microtubules / metabolism
  • Models, Biological
  • Models, Genetic
  • Mutation
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism
  • Protein Kinases / physiology*
  • Protein Serine-Threonine Kinases / metabolism*
  • RNA-Binding Proteins / metabolism
  • RNA-Binding Proteins / physiology*

Substances

  • Caenorhabditis elegans Proteins
  • RNA-Binding Proteins
  • SZY-20 protein, C elegans
  • Protein Kinases
  • zyg-1 protein, C elegans
  • Protein Serine-Threonine Kinases