A rapid molecular method for characterising bacterial bioburden in chronic wounds

J Wound Care. 2008 Dec;17(12):513-6. doi: 10.12968/jowc.2008.17.12.31769.

Abstract

Polymerase chain reaction is a culture-free method of identifying pathogens. This case study describes how, unlike traditional culture reports, a modification of this technique rapidly determined that Pseudomonas spp. was present in a chronic wound.

Publication types

  • Case Reports
  • Review

MeSH terms

  • Aged
  • Bacterial Typing Techniques / methods*
  • Biofilms / growth & development
  • Chronic Disease
  • Colony Count, Microbial / methods
  • DNA, Bacterial / analysis
  • Humans
  • Leg Ulcer / microbiology*
  • Male
  • Polymerase Chain Reaction / methods*
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / isolation & purification
  • Skin Care
  • Time Factors
  • Wound Infection / microbiology*

Substances

  • DNA, Bacterial