Results of a comparative study of growth properties of elaborated nutrient medium VLACON and traditional Lovenshtein-Jensen medium are presented in the paper. It is established, that the rate of mycobacterium growth on VLACON medium is 10-20 times higher than on the traditional nutrient medium. Mycobacteria preserve their tinctorial, cultural and pathogenic properties during the cultivation on VLACON medium. Besides, the possibility of use of agglutination reaction with further coloring and microscopy of agglutinates for the accelerated differentiation of human and bull mycobacterium species from the bird species and atypical mycobacteria is shown.