Synthesis of S-[13N]nitrosoglutathione (13N-GSNO) as a new potential PET imaging agent

Appl Radiat Isot. 2009 Jan;67(1):95-9. doi: 10.1016/j.apradiso.2008.09.014. Epub 2008 Oct 15.

Abstract

In the present paper, a fast and reproducible method for the synthesis of S-[(13)N]nitrosoglutathione is reported for the first time. The labeling strategy is based on the production of [(13)N]NO(3)(-) via the (16)O(p,alpha)(13)N nuclear reaction in water, as opposed to the standardized production of [(13)N]NH(4)(+) in 2mM aqueous ethanol. Following the reduction of [(13)N]NO(3)(-) to [(13)N]NO(2)(-), the reaction with glutathione in the presence of hydrochloric acid led to the desired radiotracer with a good radiochemical yield (24.2+/-2.0% end of synthesis, n=5) in a short production time (3min from the end of bombardment).

MeSH terms

  • Isotope Labeling / methods*
  • Nitrogen Radioisotopes*
  • Positron-Emission Tomography / methods*
  • Radiopharmaceuticals / chemical synthesis*
  • S-Nitrosoglutathione* / chemical synthesis
  • Solutions
  • Water

Substances

  • Nitrogen Radioisotopes
  • Radiopharmaceuticals
  • Solutions
  • Water
  • S-Nitrosoglutathione