Frequent inactivation of RUNX3 by promoter hypermethylation and protein mislocalization in oral squamous cell carcinomas

J Cancer Res Clin Oncol. 2009 May;135(5):739-47. doi: 10.1007/s00432-008-0508-x. Epub 2008 Nov 18.

Abstract

Purpose: RUNX3 is a functionally important component in transforming growth factor-beta (TGF-beta) mediated signaling pathway. Epigenetic silencing expression of RUNX3, as well as aberrant cytoplasmic retention of RUNX3 protein are causally involved in gastric carcinogenesis. Here, we examined the expression of RUNX3 gene and protein in oral squamous cell carcinomas (OSCCs) and analyzed the methylation status of RUNX3 promoter region.

Methods: About 10 normal oral mucosa and 30 OSCCs were collected to examine RUNX3 expression by RT-PCR analysis and immunohistochemistry assay using anti-RUNX3 monoclonal antibody R3-6E9. Methylation-specific PCR was carried out on the same specimens to analyze the methylation status of RUNX3 promoter. In addition, the stored paraffin-embedded specimens, including 40 oral leucoplakia (OLK) and 120 OSCCs, were examined by immunohistochemistry assay.

Results: RUNX3 gene and protein were underexpressed in OSCCs due to promoter hypermethylation. Protein mislocalization occurred frequently. Both downregulation of RUNX3 protein expression (P = 0.001) and protein mislocalization (P = 0.001) were correlated with the differentiation grades in OSCCs.

Conclusions: RUNX3 plays an important role in oral carcinogenesis. It may be a useful diagnostic marker and a potential therapeutic target for OSCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Core Binding Factor Alpha 3 Subunit / genetics*
  • DNA Methylation
  • DNA Primers
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Down-Regulation*
  • Female
  • Gene Amplification
  • Gene Silencing*
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / pathology
  • Promoter Regions, Genetic*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Reference Values
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Core Binding Factor Alpha 3 Subunit
  • DNA Primers
  • DNA, Neoplasm
  • RNA, Neoplasm
  • Runx3 protein, human