Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR

Sepehr N Tabrizi; Matthew P Stevens; Marina T van Leeuwen; Claire M Vajdic; Leon P Botes; Gabriele Medley; Richard Hillman; Andrew Grulich; Suzanne M Garland

doi:10.1128/JCM.01518-08

Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR

J Clin Microbiol. 2009 Jan;47(1):227-9. doi: 10.1128/JCM.01518-08. Epub 2008 Nov 12.

Authors

Sepehr N Tabrizi¹, Matthew P Stevens, Marina T van Leeuwen, Claire M Vajdic, Leon P Botes, Gabriele Medley, Richard Hillman, Andrew Grulich, Suzanne M Garland

Affiliation

¹ Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. sepehr.tabrizi@rch.org.au

Abstract

Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.

Publication types

Evaluation Study

MeSH terms

DNA, Viral / genetics
Humans
Male
Papillomaviridae / classification*
Papillomaviridae / genetics
Papillomaviridae / isolation & purification*
Papillomavirus Infections / diagnosis*
Papillomavirus Infections / virology
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Specimen Handling / methods*

Substances

DNA, Viral