Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells

M Shibahara; X Zhao; Y Wakamatsu; N Nomura; T Nakahara; C Jin; H Nagaso; T Murata; K K Yokoyama

doi:10.1023/A:1008155111024

Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells

Cytotechnology. 2000 Jul;33(1-3):247-51. doi: 10.1023/A:1008155111024.

Authors

M Shibahara¹, X Zhao, Y Wakamatsu, N Nomura, T Nakahara, C Jin, H Nagaso, T Murata, K K Yokoyama

Affiliation

¹ Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.

Abstract

We report here that a microbial extracellular glycolipid,mannosylerythritol lipid (MEL), induces the outgrowth ofneurites from and enhances the activity of acetylcholinesterase(AChE) in PC12 pheochromocytoma cells. Furthermore, treatment ofPC12 cells with MEL increased levels of galactosylceramide(Galbeta1-1'Cer; GalCer). Exposure of PC12 cells to exogenous GalCer caused the dose-dependent outgrowth ofneurites. By contrast, treatment of PC12 cells with nerve growthfactor (NGF) did not increase the level of GalCer in the cells. The neurite-related morphological changes induced by GalCerdifferend from those induced by NGF, indicating differencesbetween the signal transduction pathways triggered by NGF and by GalCer.