Chromatin recruitment of DNA repair proteins: lessons from the fanconi anemia and double-strand break repair pathways

Mol Cell. 2008 Nov 7;32(3):306-12. doi: 10.1016/j.molcel.2008.10.009.

Abstract

In response to DNA damage, eukaryotic cells must rapidly load DNA repair proteins onto damaged chromatin. Chromatin recruitment often entails ubiquitination of a damage-specific DNA repair protein, interaction with a ubiquitin binding factor, assembly of a multisubunit DNA repair complex, and eventually a deubiquitination event once the DNA repair reaction has been completed. This review focuses on the recent discoveries in the Fanconi Anemia (FA) and DNA double-strand break (DSB) repair pathways, which underscore the importance of regulated chromatin loading in the DNA damage response. Interestingly, these two pathways share several features, suggesting a more general mechanism for DNA-repair regulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Chromatin / genetics*
  • Chromatin Assembly and Disassembly
  • DNA Breaks, Double-Stranded
  • DNA Damage*
  • DNA Repair*
  • Fanconi Anemia / genetics*
  • Fanconi Anemia / metabolism
  • Homeostasis
  • Humans
  • Ubiquitin / metabolism
  • Ubiquitin-Activating Enzymes / genetics
  • Ubiquitin-Activating Enzymes / metabolism
  • Ubiquitination

Substances

  • Chromatin
  • Ubiquitin
  • Ubiquitin-Activating Enzymes