Abstract
Depletion of CD4+ cells using anti-CD4 monoclonal antibodies leads to allograft tolerance. Here we show that anti-CD4-mediated tolerance to pancreatic islets of Langerhans transplanted from an A/J (IEk) donor to a diabetic C57B1/6 (B6) (IE-) recipient occurs in the absence of clonal deletion of the potentially IE-reactive V beta 11+ T cells. Instead, a state of clonal anergy is induced in both the CD4+V beta 11+ and CD8+V beta 11+ T cell subsets. This clonal anergy can be partially overcome in vitro by the addition of recombinant interleukin 2.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte / immunology
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CD4 Antigens / immunology*
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CD8 Antigens
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Graft Survival / immunology
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Immune Tolerance / immunology*
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Islets of Langerhans Transplantation / immunology*
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Lymphocyte Activation / immunology
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Male
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Mice
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Mice, Inbred A
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Mice, Inbred C57BL
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Receptors, Antigen, T-Cell / immunology
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T-Lymphocyte Subsets / immunology
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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CD4 Antigens
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CD8 Antigens
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Receptors, Antigen, T-Cell