Glutathione oxidase-like activity of glass beads, silica gel and anion-exchange resin modified with cobalt(III)-tetrakis(4-carboxyphenyl)porphine

M Mifune; R Harada; A Iwado; N Motohashi; Y Saito

doi:10.1016/s0039-9140(98)00042-3

Glutathione oxidase-like activity of glass beads, silica gel and anion-exchange resin modified with cobalt(III)-tetrakis(4-carboxyphenyl)porphine

Talanta. 1998 Aug;46(6):1583-90. doi: 10.1016/s0039-9140(98)00042-3.

Authors

M Mifune¹, R Harada, A Iwado, N Motohashi, Y Saito

Affiliation

¹ Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 700, Japan.

PMID: 18967291
DOI: 10.1016/s0039-9140(98)00042-3

Abstract

Silica gel and glass beads were modified by using acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and physical adsorption. The carriers modified with Co(3+)-TCPP proved to accelerate the redox reaction which is catalyzed by glutathione oxidase (GSHOx), while those modified with Mn(3+)-TCPP exhibited no activity. Formation of GS-SG and hydrogen peroxide was confirmed by means of mass spectroscopy and colored reaction, respectively. The silica gel modified with Co(3+)-TCPP exhibited the strongest activity among the tested carriers, and was expected to be useful practically as a solid catalyst for the determination of glutathione.