Multicolored patterns can be fabricated by evaporating a single dye species on a prepatterned polymer substrate. The ratios of dye to polymer are different on protrusion and recess areas of the prepatterned surface, which can result in different aggregates and emissions. The polymer substrate was prepatterned using nanoimprint lithography (NIL) without any further process. This method may provide a facile route for fabricating large-area multicolored patterns.