A field study among workers in the fish processing industry (n = 143) was performed to obtain information about skin surface temperature, transepidermal water loss (TEWL), and electrical capacitance and their relationships during work. The skin temperature, TEWL and electrical capacitance were measured on the fingers, hands and forearms. A linear positive relation was found between the temperature and TEWL (in all measured areas), a linear negative relation between the temperature and capacitance (fingers and palms), and a linear negative relation between the capacitance and TEWL (fingers). The results on the fingers among workers in the fish processing industry were compared with results among metal workers (n = 52), cleaners (n = 30), gut cleaners (n = 25), nurses (n = 16), office workers with indoor climate syndrome (n = 20) and normal controls (n = 29). A linear positive relation was found between the respective temperature-TEWL values and a linear negative relation between the respective temperature-capacitance values in the various groups. Furthermore the slope of the temperature-TEWL relations was identical in all groups. Therefore, differences in TEWL levels (comparison at the same temperature) between the respective groups and controls and between the various groups might indicate damage to the skin barrier caused by contact with different irritants and chemicals. However, differences in environment-related variables in the various occupations might also affect TEWL levels. This field study demonstrates, from a practical point of view, how the skin temperature affects TEWL in the various occupations and, as a new point, how sensitive capacitance is to changes in skin temperature. Seasonal variation in TEWL and capacitance was demonstrated among workers in the fish processing industry, with a low TEWL and a high capacitance during summer when the workload is lower.