Whole genome approaches to identify early meiotic gene candidates in cereals

Funct Integr Genomics. 2009 May;9(2):219-29. doi: 10.1007/s10142-008-0097-4. Epub 2008 Oct 4.

Abstract

Early events during meiotic prophase I underpin not only viability but the variation of a species from generation to generation. Understanding and manipulating processes such as chromosome pairing and recombination are integral for improving plant breeding. This study uses comparative genetics, quantitative trait locus (QTL) analysis and a transcriptomics-based approach to identify genes that might have a role in genome-wide recombination control. Comparative genetics and the analysis of the yeast and Arabidopsis sequenced genomes has allowed the identification of early meiotic candidates that are conserved in wheat, rice and barley. Secondly, scoring recombination frequency as a phenotype for QTL analysis across wheat, rice and barley mapping populations has enabled us to identify genomic regions and candidate genes that could be involved in genome-wide recombination. Transcriptome data for candidate genes indicate that they are expressed in meiotic tissues. Candidates identified included a non-annotated expressed protein, a DNA topoisomerase 2-like candidate, RecG, RuvB and RAD54 homologues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Chromosomes, Plant / genetics
  • Conserved Sequence
  • Databases, Genetic
  • Edible Grain / classification
  • Edible Grain / cytology*
  • Edible Grain / genetics*
  • Gene Expression Profiling
  • Genome, Plant
  • Meiosis / genetics*
  • Phenotype
  • Quantitative Trait Loci
  • Recombination, Genetic
  • Species Specificity